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MED8 Polyclonal antibody, PBS Only

MED8 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12182-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

    WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

产品信息

12182-1-PBS targets MED8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen MED8 fusion protein Ag2827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator complex subunit 8
Synonyms
Calculated Molecular Weight 212 aa, 23 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC010543
Gene Symbol MED8
Gene ID (NCBI) 112950
RRIDAB_2877834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G25
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.