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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

    WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

MED8 Polyclonal antibody, PBS Only

MED8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12182-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12182-1-PBS targets MED8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 MED8 fusion protein Ag2827 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 8
别名
计算分子量 212 aa, 23 kDa
观测分子量 29 kDa
GenBank蛋白编号BC010543
基因名称 MED8
Gene ID (NCBI) 112950
RRIDAB_2877834
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96G25
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.