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  • Featured Product
  • KD/KO Validated

MEK1 Monoclonal antibody, PBS Only (Capture)

MEK1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F6D7

Cat no : 67872-1-PBS

Print datasheet

Synonyms

MAP2K1, MAPKK 1, MAP2K 1, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1

验证数据展示

  • WB analysis of A431 using 67872-1-Ig (same clone as 67872-1-PBS)

    WB analysis of A431 using 67872-1-Ig (same clone as 67872-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (MEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

    WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

    Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

    WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

    WB result of MEK1 antibody (67872-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEK1 transfected HeLa cells. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50180-1

    Cytometric bead array in cell lysate using MP50180-1

    Cytometric bead array in cell lysate using MP50180-1, Phospho-MEK1 (Thr386) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67872-1-PBS. Detection antibody: 68015-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.

产品信息

67872-1-PBS targets MEK1 as part of a matched antibody pair:

MP50180-1: 67872-1-PBS capture and 68015-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 1
Synonyms MAP2K1, MAPKK 1, MAP2K 1, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC139729
Gene Symbol MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02750
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.