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MEK2 Monoclonal antibody, PBS Only

MEK2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1H8C2

Cat No : 67410-1-PBS

Print datasheet

Synonyms

ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, PRKMK2

验证数据展示

  • WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MAP2K2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MAP2K2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2K2 antibody included
  • IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MAP2K2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2K2 antibody included
  • IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

    IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.

产品信息

67410-1-PBS targets MEK2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MEK2 fusion protein Ag29871 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 2
Synonyms ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, PRKMK2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-47 kDa
GenBank Accession NumberBC018645
Gene Symbol MAP2K2
Gene ID (NCBI) 5605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36507
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.