MEK2 Monoclonal antibody
MEK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H8C2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, Neuro-2a cells, MOLT-4 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
67410-1-Ig targets MEK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MEK2 fusion protein Ag29871 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase 2 |
| Synonyms | MAP2K2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44-47 kDa |
| GenBank Accession Number | BC018645 |
| Gene Symbol | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2882651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P36507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEK2 antibody 67410-1-Ig | Download protocol |
| IHC protocol for MEK2 antibody 67410-1-Ig | Download protocol |
| IF protocol for MEK2 antibody 67410-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Development and validation of cuproptosis-related lncRNAs associated with pancreatic cancer immune microenvironment based on single-cell | ||
Mol Cell Biochem Anti-inflammatory effects of extracellular vesicles from Morchella on LPS-stimulated RAW264.7 cells via the ROS-mediated p38 MAPK signaling pathway. | ||
Signal Transduct Target Ther IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas. | ||
Proc Natl Acad Sci U S A ERK and c-Myc signaling in host-derived tumor endothelial cells is essential for solid tumor growth | ||
Front Med 5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I |
