验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67410-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67410 targets MEK1/2 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | MEK1/2 fusion protein Ag29871 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 2 |
| 别名 | MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44-47 kDa |
| GenBank蛋白编号 | BC018645 |
| 基因名称 | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2919466 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P36507 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MEK1/2 antibody CL488-67410 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |