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验证数据展示

  • IF Staining of HeLa using CL594-67410

    IF Staining of HeLa using CL594-67410

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MEK1/2 antibody (CL594-67410, Clone: 1H8C2 ) at dilution of 1:200.

CoraLite®594-conjugated MEK1/2 Monoclonal antibody

MEK1/2 Monoclonal Antibody for IF/ICC
Cat No. CL594-67410

Print datasheet

CloneNo. 1H8C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67410-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67410 targets MEK1/2 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen MEK1/2 fusion protein Ag29871 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 2
Synonyms MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-47 kDa
GenBank Accession NumberBC018645
Gene Symbol MEK2
Gene ID (NCBI) 5605
RRIDAB_2920128
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36507
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

实验方案

Product Specific Protocols
IF protocol for CL594 MEK1/2 antibody CL594-67410Download protocol
Standard Protocols
Click here to view our Standard Protocols