CoraLite®594-conjugated MEK2 Monoclonal antibody
MEK2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1H8C2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67410-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67410 targets MEK2 in IF/ICC applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | MEK2 fusion protein Ag29871 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase kinase 2 |
Synonyms | ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, PRKMK2 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 44-47 kDa |
GenBank Accession Number | BC018645 |
Gene Symbol | MEK2 |
Gene ID (NCBI) | 5605 |
RRID | AB_2920128 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P36507 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 MEK2 antibody CL594-67410 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |