• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of MCF-7 using 82893-1-RR (same clone as 82893-1-PBS)

    FC experiment of MCF-7 using 82893-1-RR (same clone as 82893-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82893-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82893-1-RR

    Affinity and Kinetic Characterization of 82893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

MELK Recombinant antibody, PBS Only

MELK Uni-rAbTM Recombinant Antibody for FC (Intra), Indirect ELISA
Cat No. 82893-1-PBS

产品说明书

CloneNo. 230106D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), Indirect ELISA

别名

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MELK Ready-To-Use IHC Kit. MELK primary antibody included.

产品信息

82893-1-PBS targets MELK in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 MELK fusion protein Ag1897 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 maternal embryonic leucine zipper kinase
别名 KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
计算分子量 651 aa, 75 kDa
GenBank蛋白编号BC014039
基因名称 MELK
Gene ID (NCBI) 9833
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14680
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.