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MEMO1 Polyclonal antibody

MEMO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14604-1-AP

Print datasheet

Synonyms

HCV NS5A-transactivated protein 7, CGI-27, CGI27, CGI 27, C2orf4

验证数据展示

  • WB analysis using 14604-1-AP

    WB analysis using 14604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14604-1-AP (MEMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 14604-1-AP

    IF Staining of BxPC-3 using 14604-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 14604-1-AP (MEMO1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of A549 using 14604-1-AP

    WB analysis of A549 using 14604-1-AP

    WB result of MEMO1 antibody (14604-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEMO1 transfected A549 cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 14604-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 14604-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14604-1-AP (MEMO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells, A549 cells, rat testis tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14604-1-AP targets MEMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MEMO1 fusion protein Ag6151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mediator of cell motility 1
Synonyms HCV NS5A-transactivated protein 7, CGI-27, CGI27, CGI 27, C2orf4
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 31-34 kDa
GenBank Accession NumberBC070046
Gene Symbol MEMO1
Gene ID (NCBI) 51072
RRIDAB_2297647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y316
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MEMO1 antibody 14604-1-APDownload protocol
IHC protocol for MEMO1 antibody 14604-1-APDownload protocol
IF protocol for MEMO1 antibody 14604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols