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MEPCE Polyclonal antibody
MEPCE Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells, K-562 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IP | See 1 publications below |
产品信息
11526-1-AP targets MEPCE in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human |
Cited Reactivity | human, zebrafish |
Immunogen | MEPCE fusion protein Ag2092 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | methylphosphate capping enzyme |
Synonyms | Bin3 homolog, MEPCE, methylphosphate capping enzyme |
Calculated Molecular Weight | 689 aa, 74 kDa |
Observed Molecular Weight | 74 kDa |
GenBank Accession Number | BC018935 |
Gene Symbol | MEPCE |
Gene ID (NCBI) | 56257 |
RRID | AB_2143568 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q7L2J0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MEPCE antibody 11526-1-AP | Download protocol |
IHC protocol for MEPCE antibody 11526-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Dev Cell Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation. | ||
Proc Natl Acad Sci U S A 7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development. | ||
Nucleic Acids Res 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation. | ||