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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MEPCE Polyclonal antibody

MEPCE Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14917

Print datasheet

Synonyms

Bin3 homolog, FLJ20257, MEPCE, methylphosphate capping enzyme

验证数据展示

  • IF Staining of MCF-7 using CL488-14917

    IF Staining of MCF-7 using CL488-14917

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 MEPCE antibody (CL488-14917) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MEPCE Ready-To-Use IHC Kit. MEPCE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14917-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14917 targets MEPCE in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen MEPCE fusion protein Ag6733 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methylphosphate capping enzyme
Synonyms Bin3 homolog, FLJ20257, MEPCE, methylphosphate capping enzyme
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC000556
Gene Symbol MEPCE
Gene ID (NCBI) 56257
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L2J0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEPCE is a somponent of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MEPCE antibody CL488-14917Download protocol
Standard Protocols
Click here to view our Standard Protocols