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验证数据展示

  • WB analysis of HepG2 using 18804-1-AP (same clone as 18804-1-PBS)

    WB analysis of HepG2 using 18804-1-AP (same clone as 18804-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 18804-1-AP (same clone as 18804-1-PBS)

    WB analysis of HeLa using 18804-1-AP (same clone as 18804-1-PBS)

    WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MEPE Polyclonal antibody, PBS Only

MEPE Polyclonal Antibody for WB, Indirect ELISA
Cat No. 18804-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

18804-1-PBS targets MEPE in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix extracellular phosphoglycoprotein
别名
计算分子量 525 aa, 58 kDa
观测分子量 43 kDa, 98 kDa
GenBank蛋白编号BC128158
基因名称 MEPE
Gene ID (NCBI) 56955
RRIDAB_2143574
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ76
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).