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验证数据展示

  • WB analysis of HeLa using 23256-1-AP

    WB analysis of HeLa using 23256-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 23256-1-AP

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 23256-1-AP

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HeLa using 23256-1-AP

    IP experiment of HeLa using 23256-1-AP

    IP result of anti-MEPE (IP:23256-1-AP, 4ug; Detection:23256-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of HepG2 using 23256-1-AP

    IF Staining of HepG2 using 23256-1-AP

    Immunofluorescent analysis of HepG2 cells, using MEPE antibody 23256-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Cat No. 23256-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IF, IHC, ELISA

别名

MEPE, OF45, Osteoblast/osteocyte factor 45

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23256-1-AP targets MEPE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IF, IHC, ELISA Application Description
经测试反应性 human
免疫原 MEPE fusion protein Ag19758 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix extracellular phosphoglycoprotein
别名 MEPE, OF45, Osteoblast/osteocyte factor 45
计算分子量 525 aa, 58 kDa
观测分子量 98 kDa
GenBank蛋白编号BC128158
基因名称 MEPE
Gene ID (NCBI) 56955
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.

实验方案

Product Specific Protocols
WB protocol for MEPE antibody 23256-1-APDownload protocol
IHC protocol for MEPE antibody 23256-1-APDownload protocol
IF protocol for MEPE antibody 23256-1-APDownload protocol
IP protocol for MEPE antibody 23256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols