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MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23256-1-AP

Print datasheet

Synonyms

MEPE, OF45, Osteoblast/osteocyte factor 45

验证数据展示

  • WB analysis of HeLa using 23256-1-AP

    WB analysis of HeLa using 23256-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 23256-1-AP

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 23256-1-AP

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HeLa using 23256-1-AP

    IP experiment of HeLa using 23256-1-AP

    IP result of anti-MEPE (IP:23256-1-AP, 4ug; Detection:23256-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of HepG2 using 23256-1-AP

    IF Staining of HepG2 using 23256-1-AP

    Immunofluorescent analysis of HepG2 cells, using MEPE antibody 23256-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23256-1-AP is MEPE Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MEPE fusion protein Ag19758 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix extracellular phosphoglycoprotein
Synonyms MEPE, OF45, Osteoblast/osteocyte factor 45
Calculated Molecular Weight 525 aa, 58 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC128158
Gene Symbol MEPE
Gene ID (NCBI) 56955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.

实验方案

Product Specific Protocols
WB protocol for MEPE antibody 23256-1-APDownload protocol
IHC protocol for MEPE antibody 23256-1-APDownload protocol
IF protocol for MEPE antibody 23256-1-APDownload protocol
IP protocol for MEPE antibody 23256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols