验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23256-1-AP targets MEPE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IP, IF, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | MEPE fusion protein Ag19758 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | matrix extracellular phosphoglycoprotein |
Synonyms | MEPE, OF45, Osteoblast/osteocyte factor 45 |
Calculated Molecular Weight | 525 aa, 58 kDa |
Observed Molecular Weight | 98 kDa |
GenBank Accession Number | BC128158 |
Gene Symbol | MEPE |
Gene ID (NCBI) | 56955 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NQ76 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for MEPE antibody 23256-1-AP | Download protocol |
IHC protocol for MEPE antibody 23256-1-AP | Download protocol |
IF protocol for MEPE antibody 23256-1-AP | Download protocol |
IP protocol for MEPE antibody 23256-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |