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验证数据展示

  • WB analysis using 84156-2-RR

    WB analysis using 84156-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84156-2-RR (MERTK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84156-2-RR

    IHC staining of mouse kidney using 84156-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84156-2-RR (MERTK antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 84156-2-RR

    FC experiment of Jurkat using 84156-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MERTK Recombinant antibody (84156-2-RR, Clone:241252H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84156-2-RR

    Affinity and Kinetic Characterization of 84156-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84156-2-RR against Human MERTK were performed. The affinity constant is 0.444 nM.

MERTK Recombinant antibody

MERTK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84156-2-RR

产品说明书

CloneNo. 241252H3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Proto-oncogene c-Mer, Proto oncogene c Mer, MER, EC:2.7.10.1, c mer

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, HEK-293T cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84156-2-RR targets MERTK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MERTK fusion protein Ag27540 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 c-mer proto-oncogene tyrosine kinase
别名 Proto-oncogene c-Mer, Proto oncogene c Mer, MER, EC:2.7.10.1, c mer
计算分子量 999 aa, 110 kDa
观测分子量160-180 kDa
GenBank蛋白编号BC114918
基因名称 MERTK
Gene ID (NCBI) 10461
RRIDAB_3671721
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12866
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).

实验方案

Product Specific Protocols
WB protocol for MERTK antibody 84156-2-RRDownload protocol
IHC protocol for MERTK antibody 84156-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols