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MESDC2 Polyclonal antibody

MESDC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10958-1-AP

Print datasheet

Synonyms

MESDM, MESD, LRP chaperone MESD, LDLR chaperone MESD, KIAA0081

验证数据展示

  • WB analysis using 10958-1-AP

    WB analysis using 10958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

  • IHC staining of human placenta using 10958-1-AP

    IHC staining of human placenta using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 10958-1-AP

    IHC staining of human placenta using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 10958-1-AP

    IHC staining of human stomach using 10958-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10958-1-AP targets MESDC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MESDC2 fusion protein Ag1403 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mesoderm development candidate 2
Synonyms MESDM, MESD, LRP chaperone MESD, LDLR chaperone MESD, KIAA0081
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC009210
Gene Symbol MESDC2
Gene ID (NCBI) 23184
RRIDAB_2143722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14696
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MESDC2 antibody 10958-1-APDownload protocol
IHC protocol for MESDC2 antibody 10958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols