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验证数据展示

  • WB analysis of PC-3 using 60263-1-Ig

    WB analysis of PC-3 using 60263-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60263-1-Ig

    IHC staining of human prostate cancer using 60263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens).

    Ready-to-use IHC kits available with MEST antibody included
  • IHC staining of human prostate cancer using 60263-1-Ig

    IHC staining of human prostate cancer using 60263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens).

    Ready-to-use IHC kits available with MEST antibody included
  • IF Staining of BxPC-3 using 60263-1-Ig

    IF Staining of BxPC-3 using 60263-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

MEST Monoclonal antibody

MEST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60263-1-Ig

产品说明书

CloneNo. 1A4A1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy MEST Ready-To-Use IHC Kit. MEST primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60263-1-Ig targets MEST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MEST fusion protein Ag11929 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mesoderm specific transcript homolog (mouse)
别名 1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1
计算分子量 38 kDa
观测分子量 34 kDa
GenBank蛋白编号BC011908
基因名称 MEST
Gene ID (NCBI) 4232
RRIDAB_2881384
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5EB52
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

实验方案

Product Specific Protocols
WB protocol for MEST antibody 60263-1-IgDownload protocol
IHC protocol for MEST antibody 60263-1-IgDownload protocol
IF protocol for MEST antibody 60263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols