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MEST Monoclonal antibody, PBS Only

MEST Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4A1

Cat no : 60263-1-PBS

Print datasheet

Synonyms

MEST, PEG1

验证数据展示

  • WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MEST antibody included
  • IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MEST antibody included
  • IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MEST Ready-To-Use IHC Kit. MEST primary antibody included.

产品信息

60263-1-PBS targets MEST in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen MEST fusion protein Ag11929 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mesoderm specific transcript homolog (mouse)
Synonyms MEST, PEG1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC011908
Gene Symbol MEST
Gene ID (NCBI) 4232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5EB52
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.