验证数据展示

WB analysis using 25869-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 25869-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-Met (Cytoplasmic) (25869-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of HepG2 using 25869-1-AP
WB result of c-Met (Cytoplasmic) antibody (25869-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met (Cytoplasmic) transfected HepG2 cells.
IHC staining of human lung cancer using 25869-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MET antibody included
IHC staining of human lung cancer using 25869-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MET antibody included
IP experiment of HeLa using 25869-1-AP
IP result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.
IF Staining of HepG2 using 25869-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using c-Met (Cytoplasmic) antibody (25869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

c-Met (Cytoplasmic) Polyclonal antibody

c-Met (Cytoplasmic) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 25869-1-AP

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Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Synonyms

c-Met, MET, HGF/SF receptor, HGF receptor, EC:2.7.10.1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (53)
实验方案

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MSDS

经过测试的应用

Positive WB detected in	mouse liver tissue, HepG2 cells, MDCK cells, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy MET Ready-To-Use IHC Kit. MET primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 8 publications below
WB	See 39 publications below
IHC	See 13 publications below
IF	See 3 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

产品信息

25869-1-AP targets c-Met (Cytoplasmic) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat
Immunogen	c-Met (Cytoplasmic) fusion protein Ag23140 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	met proto-oncogene (hepatocyte growth factor receptor)
Synonyms	c-Met, MET, HGF/SF receptor, HGF receptor, EC:2.7.10.1
Calculated Molecular Weight	1390 aa, 155 kDa
Observed Molecular Weight	145 kDa
GenBank Accession Number	BC130420
Gene Symbol	MET
Gene ID (NCBI)	4233
RRID	AB_2880276
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08581
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

实验方案

Product Specific Protocols
WB protocol for c-Met (Cytoplasmic) antibody 25869-1-AP	Download protocol
IHC protocol for c-Met (Cytoplasmic) antibody 25869-1-AP	Download protocol
IF protocol for c-Met (Cytoplasmic) antibody 25869-1-AP	Download protocol
IP protocol for c-Met (Cytoplasmic) antibody 25869-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nat Metab LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration Authors - Yalan Deng View Article
rat	WB	Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy Authors - Ji-Chao Wu View Article
mouse	WB	Clin Transl Med Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway. Authors - Xi Dong View Article
	WB	Oxid Med Cell Longev PMID: 31781338 Authors - Ya-Dong Liu View Article
human	IHC	Mol Ther Nucleic Acids miRNA in food simultaneously controls animal viral disease and human tumorigenesis. Authors - Yalei Cui View Article
human	WB	Mol Ther Nucleic Acids Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer. Authors - Yalei Cui View Article

验证数据展示

WB analysis using 25869-1-AP

FC experiment of HeLa using 25869-1-AP

WB analysis of HepG2 using 25869-1-AP

IHC staining of human lung cancer using 25869-1-AP

IHC staining of human lung cancer using 25869-1-AP

IP experiment of HeLa using 25869-1-AP

IF Staining of HepG2 using 25869-1-AP

c-Met (Cytoplasmic) Polyclonal antibody

c-Met (Cytoplasmic) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

PMID: 31781338

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.