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c-Met (Cytoplasmic) Polyclonal antibody

c-Met (Cytoplasmic) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 25869-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

c-Met, MET, HGF/SF receptor, HGF receptor, EC:2.7.10.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, MDCK cells, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MET Ready-To-Use IHC Kit. MET primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25869-1-AP targets c-Met (Cytoplasmic) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse, rat
免疫原 c-Met (Cytoplasmic) fusion protein Ag23140 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 met proto-oncogene (hepatocyte growth factor receptor)
别名 c-Met, MET, HGF/SF receptor, HGF receptor, EC:2.7.10.1
计算分子量 1390 aa, 155 kDa
观测分子量 145 kDa
GenBank蛋白编号BC130420
基因名称 MET
Gene ID (NCBI) 4233
RRIDAB_2880276
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08581
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

实验方案

Product Specific Protocols
WB protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
IHC protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
IF protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
IP protocol for c-Met (Cytoplasmic) antibody 25869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Metab

LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration

Authors - Yalan Deng
ratWB

Acta Pharmacol Sin

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Authors - Ji-Chao Wu
mouseWB

Clin Transl Med

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Authors - Xi Dong
WB

Oxid Med Cell Longev

PMID: 31781338

Authors - Ya-Dong Liu
humanIHC

Mol Ther Nucleic Acids

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Authors - Yalei Cui
humanWB

Mol Ther Nucleic Acids

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Authors - Yalei Cui
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