验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-25869 targets c-Met (Cytoplasmic) in FC (Intra) applications and shows reactivity with human, canine, mouse, rat samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human, canine, mouse, rat |
免疫原 | c-Met (Cytoplasmic) fusion protein Ag23140 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | met proto-oncogene (hepatocyte growth factor receptor) |
别名 | MET, c-Met, c-Met (Cytoplasmic), EC:2.7.10.1, HGF receptor, HGF/SF receptor, HGFR, AUTS9, c Met, Proto oncogene c Met, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met, Tyrosine-protein kinase Met |
计算分子量 | 1390 aa, 155 kDa |
观测分子量 | 145 kDa |
GenBank蛋白编号 | BC130420 |
基因名称 | MET |
Gene ID (NCBI) | 4233 |
RRID | AB_3084093 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P08581 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 c-Met (Cytoplasmic) antibody CL488-25869 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |