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CoraLite® Plus 488-conjugated c-Met (Cytoplasmic) Polyclonal antibody

c-Met (Cytoplasmic) Polyclonal Antibody for FC (Intra)
Cat No. CL488-25869

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine, mouse, rat

应用

FC (Intra)

MET, c-Met, c-Met (Cytoplasmic), EC:2.7.10.1, HGF receptor, HGF/SF receptor, HGFR, AUTS9, c Met, Proto oncogene c Met, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met, Tyrosine-protein kinase Met

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MET Ready-To-Use IHC Kit. MET primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25869-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25869 targets c-Met (Cytoplasmic) in FC (Intra) applications and shows reactivity with human, canine, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, canine, mouse, rat
免疫原 c-Met (Cytoplasmic) fusion protein Ag23140 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 met proto-oncogene (hepatocyte growth factor receptor)
别名 MET, c-Met, c-Met (Cytoplasmic), EC:2.7.10.1, HGF receptor, HGF/SF receptor, HGFR, AUTS9, c Met, Proto oncogene c Met, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met, Tyrosine-protein kinase Met
计算分子量 1390 aa, 155 kDa
观测分子量 145 kDa
GenBank蛋白编号BC130420
基因名称 MET
Gene ID (NCBI) 4233
RRIDAB_3084093
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08581
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 c-Met (Cytoplasmic) antibody CL488-25869Download protocol
Standard Protocols
Click here to view our Standard Protocols
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