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验证数据展示

  • WB analysis using 68501-1-Ig

    WB analysis using 68501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68501-1-Ig (METTL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68501-1-Ig

    WB analysis using 68501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68501-1-Ig (METTL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

METTL1 Monoclonal antibody

METTL1 Monoclonal Antibody for WB, ELISA
Cat No. 68501-1-Ig

Print datasheet

CloneNo. 2C3G7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, ELISA

Synonyms

C12orf1, FLJ95748, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells
Planning an IHC experiment? We recommend our IHCeasy METTL1 Ready-To-Use IHC Kit. METTL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68501-1-Ig targets METTL1 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, pig
Immunogen METTL1 fusion protein Ag7055 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name methyltransferase like 1
Synonyms C12orf1, FLJ95748, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w
Calculated Molecular Weight 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC000550
Gene Symbol METTL1
Gene ID (NCBI) 4234
RRIDAB_3085212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBP6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

实验方案

Product Specific Protocols
WB protocol for METTL1 antibody 68501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols