METTL1 Monoclonal antibody

METTL1 Monoclonal Antibody for WB, ELISA
Cat No. 68501-1-Ig

产品说明书

CloneNo. 2C3G7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, pig

应用

WB, ELISA

C12orf1, FLJ95748, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells
Planning an IHC experiment? We recommend our IHCeasy METTL1 Ready-To-Use IHC Kit. METTL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68501-1-Ig targets METTL1 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, pig
免疫原 METTL1 fusion protein Ag7055 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 methyltransferase like 1
别名 C12orf1, FLJ95748, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w
计算分子量 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC000550
基因名称 METTL1
Gene ID (NCBI) 4234
RRIDAB_3085212
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

实验方案

Product Specific Protocols
WB protocol for METTL1 antibody 68501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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