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验证数据展示

  • WB analysis using 84272-1-RR

    WB analysis using 84272-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84272-1-RR (METTL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 84272-1-RR

    IF Staining of A549 using 84272-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using METTL1 antibody (84272-1-RR, Clone: 241108G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 84272-1-RR

    FC experiment of A431 using 84272-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug METTL1 Recombinant antibody (84272-1-RR, Clone:241108G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84272-1-RR

    Affinity and Kinetic Characterization of 84272-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84272-1-RR against Human METTL1 were performed. The affinity constant is 0.526 nM.

METTL1 Recombinant antibody

METTL1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84272-1-RR

产品说明书

CloneNo. 241108G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

241108G8, C12orf1, EC:2.1.1.-, EC:2.1.1.33, methyltransferase like 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Caco-2 cells, HuH-7 cells, mouse brain tissue
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy METTL1 Ready-To-Use IHC Kit. METTL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84272-1-RR targets METTL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 METTL1 fusion protein Ag7055 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 1
别名 241108G8, C12orf1, EC:2.1.1.-, EC:2.1.1.33, methyltransferase like 1
计算分子量 31 kDa
观测分子量31-35 kDa
GenBank蛋白编号BC000550
基因名称 METTL1
Gene ID (NCBI) 4234
RRIDAB_3671818
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

实验方案

Product Specific Protocols
WB protocol for METTL1 antibody 84272-1-RRDownload protocol
IF protocol for METTL1 antibody 84272-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols