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METTL1 Recombinant antibody

METTL1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241108G8

Cat no : 84272-1-RR

Print datasheet

Synonyms

Methyltransferase-like protein 1, methyltransferase like 1, EC:2.1.1.33, EC:2.1.1.-, C12orf1

验证数据展示

  • WB analysis using 84272-1-RR

    WB analysis using 84272-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84272-1-RR (METTL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84272-1-RR

    FC experiment of A431 using 84272-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug METTL1 Recombinant antibody (84272-1-RR, Clone:241108G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84272-1-RR

    Affinity and Kinetic Characterization of 84272-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84272-1-RR against Human METTL1 were performed. The affinity constant is 0.526 nM.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Caco-2 cells, HuH-7 cells, mouse brain tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy METTL1 Ready-To-Use IHC Kit. METTL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84272-1-RR is METTL1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen METTL1 fusion protein Ag7055 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methyltransferase like 1
Synonyms Methyltransferase-like protein 1, methyltransferase like 1, EC:2.1.1.33, EC:2.1.1.-, C12orf1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight31-35 kDa
GenBank Accession NumberBC000550
Gene Symbol METTL1
Gene ID (NCBI) 4234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBP6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

实验方案

Product Specific Protocols
WB protocol for METTL1 antibody 84272-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols