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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated METTL5 Polyclonal antibody

METTL5 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16791

Print datasheet

Synonyms

FLJ10459, HSPC133, methyltransferase like 5, METTL5

验证数据展示

  • IF Staining of HepG2 using CL488-16791

    IF Staining of HepG2 using CL488-16791

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 METTL5 antibody (CL488-16791) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy METTL5 Ready-To-Use IHC Kit. METTL5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16791-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16791 targets METTL5 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen METTL5 fusion protein Ag10232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 5
Synonyms FLJ10459, HSPC133, methyltransferase like 5, METTL5
Calculated Molecular Weight 209 aa, 24 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC000921
Gene Symbol METTL5
Gene ID (NCBI) 29081
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRN9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 METTL5 antibody CL488-16791Download protocol
Standard Protocols
Click here to view our Standard Protocols