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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

    WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN1 antibody included
  • IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN1 antibody included
  • IF Staining of HepG2 using 66776-1-Ig (same clone as 66776-1-PBS)

    IF Staining of HepG2 using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

MFN1 Monoclonal antibody, PBS Only

MFN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66776-1-PBS

产品说明书

CloneNo. 3F11C11

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MFN1 Ready-To-Use IHC Kit. MFN1 primary antibody included.

产品信息

66776-1-PBS targets MFN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MFN1 fusion protein Ag4890 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mitofusin 1
别名 DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1
计算分子量 741 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC040557
基因名称 MFN1
Gene ID (NCBI) 55669
RRIDAB_2882122
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWA4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).