MFN1 Monoclonal antibody

MFN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66776-1-Ig

产品说明书

CloneNo. 3F11C11

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (1)

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, U-251 cells, U-87 MG cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MFN1 Ready-To-Use IHC Kit. MFN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66776-1-Ig targets MFN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 MFN1 fusion protein Ag4890 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mitofusin 1
别名 DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1
计算分子量 741 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC040557
基因名称 MFN1
Gene ID (NCBI) 55669
RRIDAB_2882122
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWA4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

实验方案

Product Specific Protocols
WB protocol for MFN1 antibody 66776-1-IgDownload protocol
IHC protocol for MFN1 antibody 66776-1-IgDownload protocol
IF protocol for MFN1 antibody 66776-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects.

Authors - Gee Euhn Choi
pigIF

Ecotoxicol Environ Saf

Zinc deficiency compromises the maturational competence of porcine oocyte by inducing mitophagy and apoptosis

Authors - Xin-Le Lai
pigIF

J Cell Physiol

DMT1 Maintains Iron Homeostasis to Regulate Mitochondrial Function in Porcine Oocytes

Authors - Jin-Xin Zhang
humanWB

Molecules

Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.

Authors - Jiling Feng
ratIHC

J Mol Histol

Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats

Authors - Fan Wang
human,ratWB

Phytomedicine

Xiangsha Liujunzi Decoction improves gastrointestinal motility in functional dyspepsia with spleen deficiency syndrome by restoring mitochondrial quality control homeostasis

Authors - Jiaqi Zhang
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