• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of T-47D using CL594-66776

    IF Staining of T-47D using CL594-66776

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using CoraLite®594-conjugated MFN1 antibody (CL594-66776, Clone: 3F11C11 ) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-66776

    IF Staining of HepG2 using CL594-66776

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 MFN1 antibody (CL594-66776, Clone: 3F11C11 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66776

    FC experiment of HepG2 using CL594-66776

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MFN1 (CL594-66776, Clone:3F11C11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated MFN1 Monoclonal antibody

MFN1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66776

产品说明书

CloneNo. 3F11C11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

mitofusin 1, hfzo2, hfzo1, Fzo homolog, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells, T-47D cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MFN1 Ready-To-Use IHC Kit. MFN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66776-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66776 targets MFN1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 MFN1 fusion protein Ag4890 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mitofusin 1
别名 mitofusin 1, hfzo2, hfzo1, Fzo homolog, EC:3.6.5.-
计算分子量 741 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC040557
基因名称 MFN1
Gene ID (NCBI) 55669
RRIDAB_2883626
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWA4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

实验方案

Product Specific Protocols
IF protocol for CL594 MFN1 antibody CL594-66776Download protocol
FC protocol for CL594 MFN1 antibody CL594-66776Download protocol
Standard Protocols
Click here to view our Standard Protocols