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CoraLite®594-conjugated MFN1 Monoclonal antibody

MFN1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F11C11

Cat no : CL594-66776

Print datasheet

Synonyms

mitofusin 1, hfzo2, hfzo1, Fzo homolog, EC:3.6.5.-

验证数据展示

  • IF Staining of T-47D using CL594-66776

    IF Staining of T-47D using CL594-66776

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using CoraLite®594-conjugated MFN1 antibody (CL594-66776, Clone: 3F11C11 ) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-66776

    IF Staining of HepG2 using CL594-66776

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 MFN1 antibody (CL594-66776, Clone: 3F11C11 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66776

    FC experiment of HepG2 using CL594-66776

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MFN1 (CL594-66776, Clone:3F11C11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells, T-47D cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66776-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66776 targets MFN1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen MFN1 fusion protein Ag4890 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mitofusin 1
Synonyms mitofusin 1, hfzo2, hfzo1, Fzo homolog, EC:3.6.5.-
Calculated Molecular Weight 741 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC040557
Gene Symbol MFN1
Gene ID (NCBI) 55669
RRIDAB_2883626
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWA4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

实验方案

Product Specific Protocols
IF protocol for CL594 MFN1 antibody CL594-66776Download protocol
FC protocol for CL594 MFN1 antibody CL594-66776Download protocol
Standard Protocols
Click here to view our Standard Protocols