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CoraLite®594-conjugated MFN2 Monoclonal antibody

MFN2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67487

产品说明书

CloneNo. 5F3B3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

IF/ICC, FC (Intra)

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MFN2 Ready-To-Use IHC Kit. MFN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67487-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67487 targets MFN2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 MFN2 fusion protein Ag29873 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mitofusin 2
别名 CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2
计算分子量 757 aa, 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC017061
基因名称 MFN2
Gene ID (NCBI) 9927
ENSEMBL Gene IDENSG00000116688
RRIDAB_2920145
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95140
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

实验方案

Product Specific Protocols
IF protocol for CL594 MFN2 antibody CL594-67487Download protocol
FC protocol for CL594 MFN2 antibody CL594-67487Download protocol
Standard Protocols
Click here to view our Standard Protocols
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