验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, human brain tissue, human kidney tissue, human liver cancer tissue, human saliva |
| Positive IHC detected in | human gliomas tissue, human brain tissue, human lung tissue, human heart tissue, human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60055-1-Ig targets MGP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | MGP fusion protein Ag1091 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | matrix Gla protein |
| 别名 | GIG36, MGLAP, matrix Gla protein, Cell growth-inhibiting gene 36 protein, 1A1C3 |
| 计算分子量 | 103 aa, 13 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC005272 |
| 基因名称 | MGP |
| Gene ID (NCBI) | 4256 |
| RRID | AB_2143330 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08493 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MGP antibody 60055-1-Ig | Download protocol |
| IHC protocol for MGP antibody 60055-1-Ig | Download protocol |
| IF protocol for MGP antibody 60055-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Inflamm Res circ_TGFBR2 Inhibits Vascular Smooth Muscle Cells Phenotypic Switch and Suppresses Aortic Dissection Progression by Sponging miR-29a | ||
J Biol Chem Reduced APPL1 impairs osteogenic differentiation of mesenchymal stem cells by facilitating MGP expression to disrupt the BMP2 pathway in osteoporosis
| ||
Int Immunopharmacol MGP+ and IDO1+ tumor-associated macrophages facilitate immunoresistance in breast cancer revealed by single-cell RNA sequencing | ||
Adv Sci (Weinh) Mgp High-Expressing MSCs Orchestrate the Osteoimmune Microenvironment of Collagen/Nanohydroxyapatite-Mediated Bone Regeneration | ||
Nat Commun Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces |
