CoraLite® Plus 405 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)
MHC Class II (I-A/I-E) Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 405 Fluorescent Dye
CloneNo.
M5/114.15.2
验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65122-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65122 targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | Activated C57BL/6 mouse spleen cells 种属同源性预测 |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | histocompatibility 2, class II antigen A, alpha |
| Synonyms | Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II |
| GenBank Accession Number | BC031711 |
| Gene Symbol | MHC Class II |
| Gene ID (NCBI) | 14960 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P14434 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 MHC Class II (I-A/I-E) antibody CL405-65122 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |