FITC Plus Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC
Cat No. FITC-65122

产品说明书

CloneNo. M5/114.15.2

宿主/亚型

Rat / IgG2b, kappa

种属反应性

Mouse and More (1)

应用

FC

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 3 publications below

产品信息

FITC-65122 targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with Mouse samples.

经测试应用 FC Application Description
文献引用应用FC
经测试反应性 Mouse
文献引用反应性mouse
免疫原 Activated C57BL/6 mouse spleen cells 种属同源性预测
宿主/亚型 Rat / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 histocompatibility 2, class II antigen A, alpha
别名 Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II
GenBank蛋白编号BC031711
基因名称 MHC Class II
Gene ID (NCBI) 14960
RRIDAB_2883788
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP14434
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

实验方案

Product Specific Protocols
FC protocol for FITC Plus MHC Class II (I-A/I-E) antibody FITC-65122Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Cell Chem Biol

Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism

Authors - Ling Huang
mouseFC

Adv Healthc Mater

Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization

Authors - Hongping Wan
mouseFC

Int J Biol Macromol

Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization

Authors - Kai Deng
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