验证数据展示
经过测试的应用
Positive FC detected in | mouse splenocytes |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 3 publications below |
产品信息
FITC-65122 targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with Mouse samples.
经测试应用 | FC Application Description |
文献引用应用 | FC |
经测试反应性 | Mouse |
文献引用反应性 | mouse |
免疫原 | Activated C57BL/6 mouse spleen cells 种属同源性预测 |
宿主/亚型 | Rat / IgG2b, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | histocompatibility 2, class II antigen A, alpha |
别名 | Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II |
GenBank蛋白编号 | BC031711 |
基因名称 | MHC Class II |
Gene ID (NCBI) | 14960 |
RRID | AB_2883788 |
偶联类型 | FITC Plus Fluorescent Dye |
最大激发/发射波长 | 495 nm / 524 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P14434 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).
实验方案
Product Specific Protocols | |
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FC protocol for FITC Plus MHC Class II (I-A/I-E) antibody FITC-65122 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Chem Biol Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism | ||
Adv Healthc Mater Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization | ||
Int J Biol Macromol Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization |