验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, T-47D cells, HeLa cells, human brain tissue, mouse lung tissue |
| Positive IP detected in | HeLa cells, T-47D cells |
| Positive IHC detected in | human lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14818-1-AP targets MICAL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | MICAL1 fusion protein Ag6578 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule associated monoxygenase, calponin and LIM domain containing 1 |
| 别名 | NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, [F-actin]-monooxygenase MICAL1 |
| 计算分子量 | 118 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC052983 |
| 基因名称 | MICAL1 |
| Gene ID (NCBI) | 64780 |
| RRID | AB_2143754 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TDZ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| IHC protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| IF protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| IP protocol for MICAL1 antibody 14818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.
| ||
Dev Cell Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling. | ||
J Cell Biol MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
| ||
Oncogene Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement | ||
