• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

MICAL1 Polyclonal antibody

MICAL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14818-1-AP

Print datasheet

Synonyms

NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, [F-actin]-monooxygenase MICAL1

验证数据展示

  • WB analysis of HeLa using 14818-1-AP

    WB analysis of HeLa using 14818-1-AP

    WB result of MICAL1 antibody (14818-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MICAL1 transfected HeLa cells.

  • IF Staining of HeLa using 14818-1-AP

    IF Staining of HeLa using 14818-1-AP

    IF result of MICAL1 antibody (14818-1-AP) with Hela Cells (Methanol fixation) MICAL1 in Red (endogenous) and BioGFP-MICAL1-FL overexpression. Courtesy of Qingyang Liu, Utrecht University.

  • WB analysis of Jurkat using 14818-1-AP

    WB analysis of Jurkat using 14818-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14818-1-AP

    IP experiment of HeLa using 14818-1-AP

    IP result of anti-MICAL1 (IP:14818-1-AP, 5ug; Detection:14818-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • IHC staining of human lung using 14818-1-AP

    IHC staining of human lung using 14818-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 14818-1-AP

    IHC staining of human lung using 14818-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of A549 using 14818-1-AP

    IF Staining of A549 using 14818-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MICAL1 antibody (14818-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, T-47D cells, HeLa cells, human brain tissue, mouse lung tissue
Positive IP detected inHeLa cells, T-47D cells
Positive IHC detected inhuman lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14818-1-AP targets MICAL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen MICAL1 fusion protein Ag6578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 1
Synonyms NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, [F-actin]-monooxygenase MICAL1
Calculated Molecular Weight 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC052983
Gene Symbol MICAL1
Gene ID (NCBI) 64780
RRIDAB_2143754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

实验方案

Product Specific Protocols
WB protocol for MICAL1 antibody 14818-1-APDownload protocol
IHC protocol for MICAL1 antibody 14818-1-APDownload protocol
IF protocol for MICAL1 antibody 14818-1-APDownload protocol
IP protocol for MICAL1 antibody 14818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.

Authors - Fengfeng Niu
  • KO Validated
View Article
humanWB

Nat Commun

Oxidation of F-actin controls the terminal steps of cytokinesis.

Authors - Stéphane Frémont
  • KD Validated
View Article
humanCoIP

Dev Cell

Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.

Authors - Jimok Yoon
View Article
humanWB

J Cell Biol

MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.

Authors - Chiara Galloni
  • KD Validated
View Article
humanIHC

Oncogene

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement

Authors - Ze Zhang
View Article

Diabetes

Semaphorin3a promotes advanced diabetic nephropathy.

Authors - Pardeep K Aggarwal
View Article