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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MICAL1 Polyclonal antibody

MICAL1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14818

Print datasheet

Synonyms

DKFZp434B1517, FLJ11937, FLJ21739, MICAL, MICAL 1, MICAL1, NICAL

验证数据展示

  • IF Staining of HeLa using CL488-14818

    IF Staining of HeLa using CL488-14818

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 MICAL1 antibody (CL488-14818) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14818-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14818 targets MICAL1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen MICAL1 fusion protein Ag6578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 1
Synonyms DKFZp434B1517, FLJ11937, FLJ21739, MICAL, MICAL 1, MICAL1, NICAL
Calculated Molecular Weight 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC052983
Gene Symbol MICAL1
Gene ID (NCBI) 64780
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDZ2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MICAL1 antibody CL488-14818Download protocol
Standard Protocols
Click here to view our Standard Protocols