CoraLite® Plus 488-conjugated MICAL1 Monoclonal antibody
MICAL1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3F5D4
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68297-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68297 targets MICAL1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | MICAL1 fusion protein Ag6578 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | microtubule associated monoxygenase, calponin and LIM domain containing 1 |
| Synonyms | DKFZp434B1517, FLJ11937, FLJ21739, MICAL, MICAL 1, MICAL1, NICAL |
| Calculated Molecular Weight | 118 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC052983 |
| Gene Symbol | MICAL1 |
| Gene ID (NCBI) | 64780 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8TDZ2 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MICAL1 antibody CL488-68297 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |