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验证数据展示

  • FC experiment of A549 using CL555-20415

    FC experiment of A549 using CL555-20415

    1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human MIF (CL555-20415) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated MIF Polyclonal antibody

MIF Polyclonal Antibody for FC (Intra)
Cat No. CL555-20415

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Synonyms

GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 555
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA549 cells
Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 20415-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-20415 targets MIF in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat, mouse
Immunogen MIF fusion protein Ag14058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase
Calculated Molecular Weight 115 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC000447
Gene Symbol MIF
Gene ID (NCBI) 4282
RRIDAB_3084532
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14174
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 MIF antibody CL555-20415Download protocol
Standard Protocols
Click here to view our Standard Protocols