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CoraLite® Plus 647-conjugated MIP-3 Beta Polyclonal antibody

MIP-3 Beta Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13397

Print datasheet

Synonyms

Beta chemokine exodus 3, C C motif chemokine 19, CCL19, CK beta 11, CKb11, EBI1 ligand chemokine, ELC, MIP 3 beta, MIP 3b, MIP-3 Beta, MIP3B, MIP-3-beta, SCYA19, Small inducible cytokine A19

验证数据展示

  • FC experiment of A549 using CL647-13397

    FC experiment of A549 using CL647-13397

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated MIP-3 Beta Polyclonal antibody (CL647-13397)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inA549 cells
Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 13397-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13397 targets MIP-3 Beta in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen MIP-3 Beta fusion protein Ag4200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) ligand 19
Synonyms Beta chemokine exodus 3, C C motif chemokine 19, CCL19, CK beta 11, CKb11, EBI1 ligand chemokine, ELC, MIP 3 beta, MIP 3b, MIP-3 Beta, MIP3B, MIP-3-beta, SCYA19, Small inducible cytokine A19
Calculated Molecular Weight 98 aa, 12 kDa
GenBank Accession NumberBC027968
Gene Symbol CCL19/MIP-3 beta
Gene ID (NCBI) 6363
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99731
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MIP-3 Beta antibody CL647-13397Download protocol
Standard Protocols
Click here to view our Standard Protocols