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验证数据展示

  • FC experiment of A549 using CL647-13397

    FC experiment of A549 using CL647-13397

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated MIP-3 Beta Polyclonal antibody (CL647-13397)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated MCCL19/MIP-3 beta Polyclonal antibody

MCCL19/MIP-3 beta Polyclonal Antibody for FC (Intra)
Cat No. CL647-13397

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

CCL19, CCL19/MIP-3 beta, MIP-3 Beta, MIP-3-beta, CCL=19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 13397-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13397 targets MCCL19/MIP-3 beta in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 MCCL19/MIP-3 beta fusion protein Ag4200 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 19
别名 CCL19, CCL19/MIP-3 beta, MIP-3 Beta, MIP-3-beta, CCL=19
计算分子量 98 aa, 12 kDa
GenBank蛋白编号BC027968
基因名称 CCL19/MIP-3 beta
Gene ID (NCBI) 6363
RRIDAB_2934885
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99731
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MCCL19/MIP-3 beta antibody CL647-13397Download protocol
Standard Protocols
Click here to view our Standard Protocols