• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated MIS12 Monoclonal antibody

MIS12 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G5F6

Cat no : CL488-67475

Print datasheet

Synonyms

Protein MIS12 homolog, MTW1, KNTC2AP, hMis12, 2G5F6

验证数据展示

  • WB analysis using CL488-67475

    WB analysis using CL488-67475

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67475 (MIS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67475

    FC experiment of HeLa using CL488-67475

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MIS12 (CL488-67475, Clone:2G5F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67475-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67475 targets MIS12 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen MIS12 fusion protein Ag16826 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name MIS12, MIND kinetochore complex component, homolog (yeast)
Synonyms Protein MIS12 homolog, MTW1, KNTC2AP, hMis12, 2G5F6
Calculated Molecular Weight 205 aa, 24 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC000229
Gene Symbol MIS12
Gene ID (NCBI) 79003
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H081
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 MIS12 antibody CL488-67475Download protocol
FC protocol for CL Plus 488 MIS12 antibody CL488-67475Download protocol
Standard Protocols
Click here to view our Standard Protocols