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MITF Recombinant antibody, PBS Only (Capture/Detector)

MITF Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240815B4

Cat no : 83803-1-PBS

Print datasheet

Synonyms

WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32

验证数据展示

  • Sandwich ELISA standard curve of MP00746-2

    Sandwich ELISA standard curve of MP00746-2

    Sandwich ELISA standard curve of MP00746-2, Human MITF Recombinant Matched Antibody Pair - PBS only. 83803-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3679. 83803-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00746-2

    Sample test of MP00746-2

    The mean MITF concentration was determined to be 3.55 ng/mL in HeLa cell extract based on a 2.7 mg/mL extract load.

  • Cytometric bead array standard curve of MP00746-1

    Cytometric bead array standard curve of MP00746-1

    Cytometric bead array standard curve of MP00746-1, MITF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83803-1-PBS. Detection antibody: 83803-2-PBS. Standard: Ag3679. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00746-2

    Cytometric bead array standard curve of MP00746-2

    Cytometric bead array standard curve of MP00746-2, MITF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83803-4-PBS. Detection antibody: 83803-1-PBS. Standard: Ag3679. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83803-1-PBS

    Affinity and Kinetic Characterization of 83803-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83803-1-PBS against Human MITF was performed. The affinity constant is below 1 pM.

产品信息

83803-1-PBS targets MITF as part of a matched antibody pair:

MP00746-1: 83803-1-PBS capture and 83803-2-PBS detection (validated in Cytometric bead array)

MP00746-2: 83803-4-PBS capture and 83803-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen MITF fusion protein Ag3679 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name microphthalmia-associated transcription factor
Synonyms WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32
Calculated Molecular Weight 91 aa, 10 kDa, 59 kDa
GenBank Accession NumberBC012503
Gene Symbol MITF
Gene ID (NCBI) 4286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75030
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C