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验证数据展示

  • FC experiment of Jurkat using CL647-13092

    FC experiment of Jurkat using CL647-13092

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MITF (CL647-13092) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated MITF Polyclonal antibody

MITF Polyclonal Antibody for FC (Intra)
Cat No. CL647-13092

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 13092-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13092 targets MITF in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MITF fusion protein Ag3679 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microphthalmia-associated transcription factor
别名 WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32
计算分子量 91 aa, 10 kDa, 59 kDa
观测分子量 59-65 kDa
GenBank蛋白编号BC012503
基因名称 MITF
Gene ID (NCBI) 4286
RRIDAB_2934879
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75030
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MITF antibody CL647-13092Download protocol
Standard Protocols
Click here to view our Standard Protocols