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CoraLite® Plus 647-conjugated MITF Polyclonal antibody

MITF Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13092

Print datasheet

Synonyms

WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32

验证数据展示

  • FC experiment of Jurkat using CL647-13092

    FC experiment of Jurkat using CL647-13092

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MITF (CL647-13092) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 13092-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13092 targets MITF in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MITF fusion protein Ag3679 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microphthalmia-associated transcription factor
Synonyms WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32
Calculated Molecular Weight 91 aa, 10 kDa, 59 kDa
Observed Molecular Weight 59-65 kDa
GenBank Accession NumberBC012503
Gene Symbol MITF
Gene ID (NCBI) 4286
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75030
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MITF antibody CL647-13092Download protocol
Standard Protocols
Click here to view our Standard Protocols