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MKL1/MRTF-A Recombinant antibody, PBS Only

MKL1/MRTF-A Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242021B4

Cat no : 84903-1-PBS

Print datasheet

Synonyms

MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438

验证数据展示

  • WB analysis using 84903-1-RR (same clone as 84903-1-PBS)

    WB analysis using 84903-1-RR (same clone as 84903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84903-1-RR (MKL1/MRTF-A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84903-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84903-1-RR

    Affinity and Kinetic Characterization of 84903-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84903-1-RR against Human MKL1/MRTF-A were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy MRTFA/MKL1 Ready-To-Use IHC Kit. MRTFA/MKL1 primary antibody included.

产品信息

84903-1-PBS targets MKL1/MRTF-A in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MKL1/MRTF-A fusion protein Ag15308 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name megakaryoblastic leukemia (translocation) 1
Synonyms MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438
Calculated Molecular Weight 931 aa, 99 kDa
Observed Molecular Weight145-160 kDa
GenBank Accession NumberBC115039
Gene Symbol MKL1
Gene ID (NCBI) 57591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ969V6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).