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验证数据展示

  • FC experiment of HeLa using 83235-1-RR (same clone as 83235-1-PBS)

    FC experiment of HeLa using 83235-1-RR (same clone as 83235-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83235-1-RR (same clone as 83235-1-PBS)

    IF Staining of MCF-7 using 83235-1-RR (same clone as 83235-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (83235-1-RR, Clone: 240045B3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83235-1-RR (same clone as 83235-1-PBS)

    FC experiment of MCF-7 using 83235-1-RR (same clone as 83235-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00216-2

    Cytometric bead array standard curve of MP00216-2

    Cytometric bead array standard curve of MP00216-2, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-4-PBS. Detection antibody: 83235-1-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83235-1-RR

    Affinity and Kinetic Characterization of 83235-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83235-1-RR against Human MKNK2 were performed. The affinity constant is 75.1 pM.

MKNK2 Recombinant antibody, PBS Only (Detector)

MKNK2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83235-1-PBS

Print datasheet

CloneNo. 240045B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83235-1-PBS targets MKNK2 as part of a matched antibody pair:

MP00216-2: 83235-4-PBS capture and 83235-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MKNK2 fusion protein Ag10546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAP kinase interacting serine/threonine kinase 2
Synonyms MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 47-52 kDa
GenBank Accession NumberBC018345
Gene Symbol MKNK2
Gene ID (NCBI) 2872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HBH9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.