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验证数据展示

  • FC experiment of HeLa using 83235-1-RR

    FC experiment of HeLa using 83235-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of MCF-7 using 83235-1-RR

    IF Staining of MCF-7 using 83235-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (83235-1-RR, Clone: 240045B3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 83235-1-RR

    FC experiment of MCF-7 using 83235-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83235-1-RR

    Affinity and Kinetic Characterization of 83235-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83235-1-RR against Human MKNK2 were performed. The affinity constant is 75.1 pM.

MKNK2 Recombinant antibody

MKNK2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83235-1-RR

Print datasheet

CloneNo. 240045B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83235-1-RR targets MKNK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MKNK2 fusion protein Ag10546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAP kinase interacting serine/threonine kinase 2
Synonyms MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 47-52 kDa
GenBank Accession NumberBC018345
Gene Symbol MKNK2
Gene ID (NCBI) 2872
RRIDAB_3670912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HBH9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.

实验方案

Product Specific Protocols
IF protocol for MKNK2 antibody 83235-1-RRDownload protocol
FC protocol for MKNK2 antibody 83235-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols