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验证数据展示

  • WB analysis using 13186-1-AP

    WB analysis using 13186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 13186-1-AP

    IHC staining of human testis using 13186-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 13186-1-AP

    IHC staining of human testis using 13186-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 13186-1-AP

    IF Staining of HepG2 using 13186-1-AP

    Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 13186-1-AP

    WB analysis using 13186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

MLF1IP Polyclonal antibody

MLF1IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13186-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CENPU, CENP-50, CENP50, CENP U(50), CENP U

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, mouse testis tissue, rat liver tissue
Positive IHC detected inhuman testis tissue, human colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13186-1-AP targets MLF1IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MLF1IP fusion protein Ag3858 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MLF1 interacting protein
别名 CENPU, CENP-50, CENP50, CENP U(50), CENP U
计算分子量 418 aa, 48 kDa
观测分子量 46-50 kDa, 60 kDa
GenBank蛋白编号BC031520
基因名称 MLF1IP
Gene ID (NCBI) 79682
RRIDAB_10642941
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ71F23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.

实验方案

Product Specific Protocols
WB protocol for MLF1IP antibody 13186-1-APDownload protocol
IHC protocol for MLF1IP antibody 13186-1-APDownload protocol
IF protocol for MLF1IP antibody 13186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Signal

Overexpression of MLF1IP promotes colorectal cancer cell proliferation through BRCA1/AKT/p27 signaling pathway.

Authors - Yuting Xu
  • KD Validated
View Article