验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, Caco-2 cells, COLO 320 cells, HEK-293 cells, Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67350-1-Ig targets MLH1 in WB, ELISA applications and shows reactivity with Human, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, rat |
免疫原 | MLH1 fusion protein Ag27723 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) |
别名 | COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1 |
计算分子量 | 756 aa, 85 kDa |
观测分子量 | 85-100 kDa, 40-45 kDa |
GenBank蛋白编号 | BC006850 |
基因名称 | MLH1 |
Gene ID (NCBI) | 4292 |
RRID | AB_2918485 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P40692 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.
实验方案
Product Specific Protocols | |
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WB protocol for MLH1 antibody 67350-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |