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MLH1 Monoclonal antibody

MLH1 Monoclonal Antibody for WB, ELISA
Cat No. 67350-1-Ig

产品说明书

CloneNo. 2F12C4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat

应用

WB, ELISA

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Caco-2 cells, COLO 320 cells, HEK-293 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67350-1-Ig targets MLH1 in WB, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, rat
免疫原 MLH1 fusion protein Ag27723 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
别名 COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1
计算分子量 756 aa, 85 kDa
观测分子量 85-100 kDa, 40-45 kDa
GenBank蛋白编号BC006850
基因名称 MLH1
Gene ID (NCBI) 4292
RRIDAB_2918485
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.

实验方案

Product Specific Protocols
WB protocol for MLH1 antibody 67350-1-IgDownload protocol
Standard Protocols
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