验证数据展示
产品信息
67350-1-PBS targets MLH1 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.
| Tested Applications | WB, Indirect ELISA Application Description |
| Tested Reactivity | Human, rat |
| Immunogen | MLH1 fusion protein Ag27723 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) |
| Synonyms | COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1 |
| Calculated Molecular Weight | 756 aa, 85 kDa |
| Observed Molecular Weight | 85-100 kDa, 40-45 kDa |
| GenBank Accession Number | BC006850 |
| Gene Symbol | MLH1 |
| Gene ID (NCBI) | 4292 |
| RRID | AB_2918485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40692 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.