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  • Featured Product
  • KD/KO Validated

MLH1 Monoclonal antibody, PBS Only

MLH1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67350-1-PBS

Print datasheet

Synonyms

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

验证数据展示

  • WB analysis using 67350-1-Ig (same clone as 67350-1-PBS)

    WB analysis using 67350-1-Ig (same clone as 67350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67350-1-Ig (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67350-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67350-1-Ig (same clone as 67350-1-PBS)

    WB analysis of HeLa using 67350-1-Ig (same clone as 67350-1-PBS)

    WB result of MLH1 antibody (67350-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells. This data was developed using the same antibody clone with 67350-1-PBS in a different storage buffer formulation.

产品信息

67350-1-PBS targets MLH1 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen MLH1 fusion protein Ag27723 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Synonyms COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1
Calculated Molecular Weight 756 aa, 85 kDa
Observed Molecular Weight 85-100 kDa, 40-45 kDa
GenBank Accession NumberBC006850
Gene Symbol MLH1
Gene ID (NCBI) 4292
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40692
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.