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  • Featured Product
  • KD/KO Validated

MLKL Monoclonal antibody, PBS Only

MLKL Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D4C6

Cat no : 66675-1-PBS

Print datasheet

Synonyms

Mixed lineage kinase domain-like protein, hMLKL, 3D4C6

验证数据展示

  • WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66675-1-Ig (MLKL antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66675-1-Ig (same clone as 66675-1-PBS)

    IF Staining of HepG2 using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody (66675-1-Ig, Clone: 3D4C6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

产品信息

66675-1-PBS targets MLKL in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityrat
Immunogen MLKL fusion protein Ag15188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mixed lineage kinase domain-like
Synonyms Mixed lineage kinase domain-like protein, hMLKL, 3D4C6
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight35-40 kDa, 50-55 kDa
GenBank Accession NumberBC028141
Gene Symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NB16
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).