验证数据展示
产品信息
66675-1-PBS targets MLKL in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | MLKL fusion protein Ag15188 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | mixed lineage kinase domain-like |
Synonyms | Mixed lineage kinase domain-like protein, hMLKL, 3D4C6 |
Calculated Molecular Weight | 471 aa, 54 kDa |
Observed Molecular Weight | 35-40 kDa, 50-55 kDa |
GenBank Accession Number | BC028141 |
Gene Symbol | MLKL |
Gene ID (NCBI) | 197259 |
RRID | AB_2882029 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8NB16 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).