• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

MLKL Monoclonal antibody, PBS Only

MLKL Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

3D4C6

Cat No : 66675-1-PBS

Print datasheet

Synonyms

FLJ34389, MLKL

验证数据展示

  • WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66675-1-Ig (MLKL antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

产品信息

66675-1-PBS targets MLKL in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen MLKL fusion protein Ag15188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mixed lineage kinase domain-like
Synonyms FLJ34389, MLKL
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight35-40kDa, 50-55 kDa
GenBank Accession NumberBC028141
Gene Symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NB16
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a prograwmmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected a 35kDa truncated isoform (PMID: 35965541).