验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MLKL Ready-To-Use IHC Kit. MLKL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66675-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
| FC | See 1 publications below |
产品信息
CL488-66675 targets MLKL in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | MLKL fusion protein Ag15188 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mixed lineage kinase domain-like |
| 别名 | Mixed lineage kinase domain-like protein, hMLKL, 3D4C6 |
| 计算分子量 | 471 aa, 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC028141 |
| 基因名称 | MLKL |
| Gene ID (NCBI) | 197259 |
| RRID | AB_2883366 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8NB16 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MLKL antibody CL488-66675 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury | ||
Int J Biol Sci Neutrophil extracellular traps trigger alveolar epithelial cell necroptosis through the cGAS-STING pathway during acute lung injury in mice |
