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MLPH Recombinant antibody

MLPH Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241621G4

Cat no : 84282-1-RR

Print datasheet

Synonyms

SLAC2A, SLAC2 A, Melanophilin, Exophilin-3, Exophilin 3

验证数据展示

  • WB analysis using 84282-1-RR

    WB analysis using 84282-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 84282-1-RR

    IHC staining of human skin cancer using 84282-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 84282-1-RR

    IHC staining of human skin cancer using 84282-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84282-1-RR

    Affinity and Kinetic Characterization of 84282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, PC-3 cells, HT-29 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84282-1-RR targets MLPH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MLPH fusion protein Ag32931 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name melanophilin
Synonyms SLAC2A, SLAC2 A, Melanophilin, Exophilin-3, Exophilin 3
Calculated Molecular Weight 66 kDa
Observed Molecular Weight66 kDa, 80 kDa
GenBank Accession NumberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

实验方案

Product Specific Protocols
WB protocol for MLPH antibody 84282-1-RRDownload protocol
IHC protocol for MLPH antibody 84282-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols