MLPH Recombinant antibody
MLPH Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
241621G4
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, LNCaP cells, MCF-7 cells, PC-3 cells, HT-29 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 84282-1-RR is MLPH Fusion Protein expressed in E. coli.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | MLPH fusion protein Ag32931 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | melanophilin |
| Synonyms | SLAC2A, SLAC2 A, Melanophilin, Exophilin-3, Exophilin 3 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa, 80 kDa |
| GenBank Accession Number | BC001653 |
| Gene Symbol | MLPH |
| Gene ID (NCBI) | 79083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BV36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MLPH antibody 84282-1-RR | Download protocol |
| IHC protocol for MLPH antibody 84282-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |