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验证数据展示

  • WB analysis using 84282-1-RR (same clone as 84282-1-PBS)

    WB analysis using 84282-1-RR (same clone as 84282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01196-3

    Sandwich ELISA standard curve of MP01196-3

    Sandwich ELISA standard curve of MP01196-3, Human MLPH Recombinant Matched Antibody Pair - PBS only. 84282-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32931. 84282-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01196-3

    Sample test of MP01196-3

    The mean MLPH concentration was determined to be 5.9 ng/mL in A549 cell extract based on a 1.5 mg/mL extract load and 1.7 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84282-1-RR

    Affinity and Kinetic Characterization of 84282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

MLPH Recombinant antibody, PBS Only (Capture)

MLPH Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84282-1-PBS

产品说明书

CloneNo. 241621G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

别名

241621G4, Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84282-1-PBS targets MLPH as part of a matched antibody pair:

MP01196-3: 84282-1-PBS capture and 84282-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 MLPH fusion protein Ag32931 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanophilin
别名 241621G4, Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A
计算分子量 66 kDa
观测分子量66 kDa, 80 kDa
GenBank蛋白编号BC001653
基因名称 MLPH
Gene ID (NCBI) 79083
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV36
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.