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验证数据展示

  • WB analysis using 67042-1-Ig

    WB analysis using 67042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67042-1-Ig (MLX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67042-1-Ig

    IHC staining of human breast cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MLX antibody included
  • IF Staining of HepG2 using 67042-1-Ig

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IF Staining of HepG2 using 67042-1-Ig

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

MLX Monoclonal antibody

MLX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67042-1-Ig

产品说明书

CloneNo. 2A8D2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MLX Ready-To-Use IHC Kit. MLX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67042-1-Ig targets MLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 MLX fusion protein Ag28729 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 MAX-like protein X
别名 bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4
计算分子量 298 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC010689
基因名称 MLX
Gene ID (NCBI) 6945
RRIDAB_2882356
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UH92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLX antibody 67042-1-IgDownload protocol
IHC protocol for MLX antibody 67042-1-IgDownload protocol
IF protocol for MLX antibody 67042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols