MLXIPL Polyclonal antibody

MLXIPL Polyclonal Antibody for WB, IP, ELISA
Cat No. 13256-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (2)

bHLHd14, CHREBP, MIO, MLX interacting protein like, MLX interactor, MLXIPL, MONDOB, WBSCR14, WS bHLH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, HepG2 cells, HeLa cells, rat liver tissue
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13256-1-AP targets MLXIPL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MLXIPL fusion protein Ag3481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MLX interacting protein-like
别名 bHLHd14, CHREBP, MIO, MLX interacting protein like, MLX interactor, MLXIPL, MONDOB, WBSCR14, WS bHLH
计算分子量 852 aa, 93 kDa
观测分子量 65 kDa
GenBank蛋白编号BC012925
基因名称 MLXIPL
Gene ID (NCBI) 51085
RRIDAB_2266665
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbohydrate response element-binding protein (ChREBP), also known as MLXIPL, is a glucose-responsive transcription factor that has a fundamental role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. Circulating blood glucose levels affect ChREBP activity in hepatocytes largely by post-translational mechanisms that include phosphorylation-dependent subcellular localization [PMID:21665952]. It enhances expression of the liver pyruvate kinase (LPK) gene and all lipogenic enzyme genes resulting in the conversion of excess dietary carbohydrate into triglycerides that can be stored as fat [PMID:15118080]. Molecular Weight of ChREBP splice variants: 62/78/91/93 kDa.

实验方案

Product Specific Protocols
WB protocol for MLXIPL antibody 13256-1-APDownload protocol
IP protocol for MLXIPL antibody 13256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
mouseWB,IHC

Clin Transl Med

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Authors - Yunqiang He
mouse

Front Pharmacol

Roxadustat, a Hypoxia-Inducible Factor 1α Activator, Attenuates Both Long- and Short-Term Alcohol-Induced Alcoholic Liver Disease.

Authors - Yongyao Gao
mouse,humanWB

J Agric Food Chem

Diosmetin Ameliorates Nonalcoholic Steatohepatitis through Modulating Lipogenesis and Inflammatory Response in a STAT1/CXCL10-Dependent Manner.

Authors - Nanxuan Luo
human,mouseWB

J Agric Food Chem

Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis.

Authors - Yan Xing
humanWB

Food Funct

Polysaccharide MCP extracted from Morchella esculenta reduces atherosclerosis in LDLR-deficient mice.

Authors - Dandan Wang
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