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MMACHC Monoclonal antibody, PBS Only

MMACHC Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4G10A11

Cat No : 66609-1-PBS

Print datasheet

Synonyms

cblC, DKFZp564I122, FLJ25671, MMACHC, RP11 291L19.3

验证数据展示

  • WB analysis of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    WB analysis of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    IF Staining of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MMACHC antibody (66609-1-Ig, Clone: 4G10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

产品信息

66609-1-PBS targets MMACHC in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen MMACHC fusion protein Ag15001 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Synonyms cblC, DKFZp564I122, FLJ25671, MMACHC, RP11 291L19.3
Calculated Molecular Weight 282 aa, 32 kDa
GenBank Accession NumberBC006122
Gene Symbol MMACHC
Gene ID (NCBI) 25974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4U1
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.