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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83188-4-RR (same clone as 83188-4-PBS)

    WB analysis using 83188-4-RR (same clone as 83188-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83188-4-RR (MMP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83188-4-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83188-4-RR (same clone as 83188-4-PBS)

    FC experiment of MCF-7 using 83188-4-RR (same clone as 83188-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-4-RR, Clone:240060A1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83188-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83188-4-RR

    Affinity and Kinetic Characterization of 83188-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-4-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

MMP13 Recombinant antibody, PBS Only

MMP13 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83188-4-PBS

产品说明书

CloneNo. 240060A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83188-4-PBS targets MMP13 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MMP13 fusion protein Ag34892 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 13
别名 Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3
计算分子量 471 aa, 54 kDa
观测分子量 70 kDa, 54 kDa
GenBank蛋白编号BC074808
基因名称 MMP13
Gene ID (NCBI) 4322
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP45452
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated form (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).